Human AZU1 (Azurocidin 1) CLIA Kit (E-CL-H0430)

Name: Human AZU1 (Azurocidin 1) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0430
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human AZU1 in samples.No significant cross-reactivity or interference between Human AZU1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	57997.000	59533.000	58765.000	58731
1000	25412.000	25622.000	25517.000	25483
500	12202.000	11708.000	11955.000	11921
250	5446.000	6182.000	5814.000	5780
125	2887.000	2799.000	2843.000	2809
62.5	1478.000	1340.000	1409.000	1375
31.25	676.000	722.000	699.000	665
0	34.000	34.000	34.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	98.610	283.650	896.670	89.680	275.150	946.340
Standard deviation	11.190	20.790	62.950	11.510	21.020	87.820
CV（%）	11.350	7.330	7.020	12.830	7.640	9.280

Recovery

The recovery of Human AZU1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	100-114	107
EDTA plasma （n=8）	94-107	100
Cell culture media （n=8）	91-105	98

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human AZU1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	92-109	100-116	89-102
1:2	Average	99	107	96
1:4	Range	95-108	86-100	91-105
1:4	Average	101	92	98
1:8	Range	101-117	91-103	104-120
1:8	Average	107	98	110

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human AZU1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human AZU1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human AZU1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human AZU1. You can calculate the concentration of Human AZU1 in the samples by comparing the RLU value of the samples to the standard curve.

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