Human BACE1 (Beta-site APP Cleaving Enzyme 1) CLIA Kit (E-CL-H0190)

Name: Human BACE1 (Beta-site APP Cleaving Enzyme 1) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0190
Price: 180.0 USD
Availability: InStock

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Human BACE1 (Beta-site APP Cleaving Enzyme 1) CLIA Kit - 1

Human BACE1 (Beta-site APP Cleaving Enzyme 1) CLIA Kit - 2

Human BACE1 (Beta-site APP Cleaving Enzyme 1) CLIA Kit - 3

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.09 ng/mL
Detection Range	0.16-10 ng/mL
Sample Volume	100 μL
Total Assay Time	4 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human BACE1 in samples.No significant cross-reactivity or interference between Human BACE1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human BACE1 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human BACE1 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	0.480	1.530	3.540	0.470	1.590	3.210
Standard deviation	0.040	0.160	0.240	0.060	0.110	0.350
CV（%）	8.330	10.460	6.780	12.770	6.920	10.900

Recovery

The recovery of Human BACE1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	84-96	90
EDTA plasma （n=8）	91-104	98
Cell culture media （n=8）	92-110	100

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human BACE1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	89-99	90-105	102-115
1:2	Average	94	96	109
1:4	Range	100-113	101-115	87-97
1:4	Average	106	109	92
1:8	Range	89-106	101-117	97-114
1:8	Average	97	109	104

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BACE1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BACE1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BACE1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human BACE1. You can calculate the concentration of Human BACE1 in the samples by comparing the RLU value of the samples to the standard curve.