Human BRAK/CXCL14 (Breast and Kidney Expressed Chemokine) CLIA Kit (E-CL-H0463)

Name: Human BRAK/CXCL14 (Breast and Kidney Expressed Chemokine) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0463
Price: 180.0 USD
Availability: InStock

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Human BRAK/CXCL14 (Breast and Kidney Expressed Chemokine) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human BRAK/CXCL14 in samples.No significant cross-reactivity or interference between Human BRAK/CXCL14 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	56144.000	65634.000	60889.000	60855
1000	24712.000	26080.000	25396.000	25362
500	12433.000	11497.000	11965.000	11931
250	4834.000	5778.000	5306.000	5272
125	2960.000	2700.000	2830.000	2796
62.5	1472.000	1290.000	1381.000	1347
31.25	689.000	777.000	733.000	699
0	34.000	34.000	34.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	103.360	176.330	819.520	106.880	177.410	873.360
Standard deviation	10.360	12.840	61.550	12.480	16.370	55.720
CV（%）	10.020	7.280	7.510	11.680	9.230	6.380

Recovery

The recovery of Human BRAK was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	94-106	100
EDTA plasma （n=8）	96-108	101
Cell culture media （n=8）	98-112	104

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human BRAK and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	100-112	99-116	86-102
1:2	Average	106	108	93
1:4	Range	99-113	100-112	85-99
1:4	Average	105	106	91
1:8	Range	97-111	84-99	96-113
1:8	Average	103	91	104

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BRAK/CXCL14. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BRAK/CXCL14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BRAK/CXCL14, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human BRAK/CXCL14. You can calculate the concentration of Human BRAK/CXCL14 in the samples by comparing the RLU value of the samples to the standard curve.

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