Human BRCA2 (Breast Cancer Susceptibility Protein 2) CLIA Kit (E-CL-H0465)

Name: Human BRCA2 (Breast Cancer Susceptibility Protein 2) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0465
Price: 180.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human BRCA2 (Breast Cancer Susceptibility Protein 2) CLIA Kit - 1

Human BRCA2 (Breast Cancer Susceptibility Protein 2) CLIA Kit - 2

Human BRCA2 (Breast Cancer Susceptibility Protein 2) CLIA Kit - 3

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	4 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human BRCA2 in samples.No significant cross-reactivity or interference between Human BRCA2 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Recovery

The recovery of Human BRCA2 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	91-101	96
EDTA plasma （n=8）	88-99	94
Cell culture media （n=8）	90-101	95

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human BRCA2 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	83-97	95-109	95-110
1:2	Average	90	102	103
1:4	Range	88-104	93-108	96-109
1:4	Average	95	101	102
1:8	Range	87-97	96-111	92-109
1:8	Average	92	102	100

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human BRCA2. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human BRCA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human BRCA2, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human BRCA2. You can calculate the concentration of Human BRCA2 in the samples by comparing the RLU value of the samples to the standard curve.