Human C1QTNF1 (C1q and Tumor Necrosis Factor Related Protein 1) CLIA Kit (E-CL-H0877)

Name: Human C1QTNF1 (C1q and Tumor Necrosis Factor Related Protein 1) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0877
Price: 180.0 USD
Availability: InStock

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Human C1QTNF1 (C1q and Tumor Necrosis Factor Related Protein 1) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.38 ng/mL
Detection Range	0.63-40 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human C1QTNF1 in samples.No significant cross-reactivity or interference between Human C1QTNF1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
40	38315.000	39335.000	38825.000	38799
20	14819.000	15987.000	15403.000	15377
10	7116.000	6674.000	6895.000	6869
5	3214.000	3342.000	3278.000	3252
2.5	1661.000	1547.000	1604.000	1578
1.25	990.000	868.000	929.000	903
0.63	579.000	609.000	594.000	568
0	25.000	27.000	26.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	2.030	5.420	14.760	2.000	5.240	15.340
Standard deviation	0.200	0.580	1.110	0.180	0.410	1.090
CV（%）	9.850	10.700	7.520	9.000	7.820	7.110

Recovery

The recovery of Human C1QTNF1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	89-103	97
EDTA plasma （n=8）	91-104	97
Cell culture media （n=8）	86-98	92

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human C1QTNF1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	91-105	88-101	88-101
1:2	Average	98	93	93
1:4	Range	98-113	101-114	87-101
1:4	Average	106	108	92
1:8	Range	87-98	92-109	88-103
1:8	Average	93	100	94

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human C1QTNF1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C1QTNF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C1QTNF1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human C1QTNF1. You can calculate the concentration of Human C1QTNF1 in the samples by comparing the RLU value of the samples to the standard curve.

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