Human C5a (Complement Component 5a) CLIA Kit (E-CL-H0174)

Name: Human C5a (Complement Component 5a) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0174
Price: 180.0 USD
Availability: InStock

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Human C5a (Complement Component 5a) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human C5a in samples.No significant cross-reactivity or interference between Human C5a and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	52332.000	58292.000	55312.000	55293
500	21536.000	26134.000	23835.000	23816
250	10982.000	9836.000	10409.000	10390
125	5168.000	5342.000	5255.000	5236
62.5	2628.000	2342.000	2485.000	2466
31.25	1418.000	1280.000	1349.000	1330
15.63	690.000	776.000	733.000	714
0	18.000	20.000	19.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	52.900	130.310	464.600	48.440	133.530	510.370
Standard deviation	5.700	9.170	44.970	4.920	14.930	38.480
CV（%）	10.780	7.040	9.680	10.160	11.180	7.540

Recovery

The recovery of Human C5a was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	96-114	104
EDTA plasma （n=8）	91-106	97
Cell culture media （n=8）	97-110	104

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human C5a and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	98-112	100-118	98-114
1:2	Average	104	108	105
1:4	Range	84-95	101-113	100-112
1:4	Average	90	107	106
1:8	Range	95-108	103-117	93-107
1:8	Average	101	109	99

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human C5a. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C5a and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C5a, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human C5a. You can calculate the concentration of Human C5a in the samples by comparing the RLU value of the samples to the standard curve.

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