Human CASP1 (Caspase 1) CLIA Kit (E-CL-H0016)

Name: Human CASP1 (Caspase 1) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0016
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	3.75 pg/mL
Detection Range	6.25-400 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human CASP1 in samples.No significant cross-reactivity or interference between Human CASP1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
400	46582.000	52664.000	49623.000	49600
200	19205.000	21889.000	20547.000	20524
100	8768.000	8472.000	8620.000	8597
50	3924.000	4510.000	4217.000	4194
25	2206.000	2190.000	2198.000	2175
12.5	1272.000	1172.000	1222.000	1199
6.25	712.000	772.000	742.000	719
0	22.000	24.000	23.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	18.810	49.910	189.930	18.270	45.540	182.670
Standard deviation	1.630	5.070	16.770	1.790	5.410	19.550
CV（%）	8.670	10.160	8.830	9.800	11.880	10.700

Recovery

The recovery of Human CASP1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	91-105	96
EDTA plasma （n=8）	90-104	97
Cell culture media （n=8）	93-106	101

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human CASP1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	100-114	97-112	89-102
1:2	Average	106	103	95
1:4	Range	94-108	86-96	86-96
1:4	Average	100	91	91
1:8	Range	95-107	98-111	92-103
1:8	Average	101	104	97

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CASP1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CASP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CASP1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human CASP1. You can calculate the concentration of Human CASP1 in the samples by comparing the RLU value of the samples to the standard curve.

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