Human CDK6 (Cyclin Dependent Kinase 6) CLIA Kit (E-CL-H0254)

Name: Human CDK6 (Cyclin Dependent Kinase 6) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0254
Price: 180.0 USD
Availability: InStock

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Human CDK6 (Cyclin Dependent Kinase 6) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human CDK6 in samples.No significant cross-reactivity or interference between Human CDK6 and analogues was observed
Recovery	80%-120%
Sample Type	Tissue homogenates, cell lysates and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	51113.000	61547.000	56330.000	56305
1000	22534.000	25608.000	24071.000	24046
500	13147.000	11085.000	12116.000	12091
250	5006.000	5790.000	5398.000	5373
125	2963.000	2745.000	2854.000	2829
62.5	1391.000	1309.000	1350.000	1325
31.25	697.000	715.000	706.000	681
0	24.000	26.000	25.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	105.940	240.960	754.580	110.830	264.010	796.920
Standard deviation	12.250	27.880	73.270	10.570	21.360	85.030
CV（%）	11.560	11.570	9.710	9.540	8.090	10.670

Recovery

The recovery of Human CDK6 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	102-117	108
EDTA plasma （n=8）	101-115	107
Cell culture media （n=8）	87-97	92

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human CDK6 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	87-102	99-113	100-117
1:2	Average	94	104	106
1:4	Range	95-110	98-110	103-116
1:4	Average	101	104	110
1:8	Range	96-109	99-111	96-112
1:8	Average	102	105	102

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CDK6. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CDK6 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CDK6, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human CDK6. You can calculate the concentration of Human CDK6 in the samples by comparing the RLU value of the samples to the standard curve.

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