Human CDNF (Cerebral Dopamine Neurotrophic Factor) CLIA Kit (E-CL-H0276)

Name: Human CDNF (Cerebral Dopamine Neurotrophic Factor) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0276
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human CDNF (Cerebral Dopamine Neurotrophic Factor) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human CDNF in samples.No significant cross-reactivity or interference between Human CDNF and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	59326.000	63058.000	61192.000	61160
1000	23914.000	24412.000	24163.000	24131
500	11264.000	11020.000	11142.000	11110
250	5077.000	5669.000	5373.000	5341
125	2741.000	2599.000	2670.000	2638
62.5	1487.000	1353.000	1420.000	1388
31.25	638.000	730.000	684.000	652
0	31.000	33.000	32.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	95.310	243.060	819.760	99.400	236.040	811.690
Standard deviation	8.700	19.150	60.250	12.640	21.550	90.260
CV（%）	9.130	7.880	7.350	12.720	9.130	11.120

Recovery

The recovery of Human CDNF was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	85-99	91
EDTA plasma （n=8）	95-112	103
Cell culture media （n=8）	86-102	93

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human CDNF and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	94-110	88-100	96-110
1:2	Average	100	93	104
1:4	Range	87-100	92-103	90-106
1:4	Average	94	98	96
1:8	Range	88-101	95-112	100-116
1:8	Average	95	102	109

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CDNF. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CDNF, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human CDNF. You can calculate the concentration of Human CDNF in the samples by comparing the RLU value of the samples to the standard curve.

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