Human CEA (Carcinoembryonic Antigen) CLIA Kit (E-CL-H0492)

Name: Human CEA (Carcinoembryonic Antigen) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0492
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human CEA (Carcinoembryonic Antigen) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human CEA/CD66 in samples.No significant cross-reactivity or interference between Human CEA/CD66 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	52871.000	63677.000	58274.000	58246
500	23772.000	23860.000	23816.000	23788
250	11135.000	9649.000	10392.000	10364
125	4681.000	5603.000	5142.000	5114
62.5	2473.000	2437.000	2455.000	2427
31.25	1391.000	1207.000	1299.000	1271
15.63	698.000	742.000	720.000	692
0	27.000	29.000	28.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	46.370	87.730	407.800	45.360	90.580	371.720
Standard deviation	4.110	6.270	29.360	4.890	6.990	24.530
CV（%）	8.860	7.150	7.200	10.780	7.720	6.600

Recovery

The recovery of Human CEA was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	98-113	105
EDTA plasma （n=8）	94-105	99
Cell culture media （n=8）	95-108	103

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human CEA and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	98-112	94-107	95-109
1:2	Average	106	100	101
1:4	Range	92-105	93-106	97-111
1:4	Average	98	99	105
1:8	Range	84-96	97-109	95-108
1:8	Average	91	104	102

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CEA/CD66. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CEA/CD66 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CEA/CD66, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human CEA/CD66. You can calculate the concentration of Human CEA/CD66 in the samples by comparing the RLU value of the samples to the standard curve.

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