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Human CGRP1(Calcitonin Gene Related Peptide 1) ELISA Kit

  • Cat.No.:E-EL-H0619

  • Reactivity: Human

To Purchase E-EL-H0619

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 15.63-1000 pg/mL
Sensitivity 9.38 pg/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human CGRP1 in samples. No significant cross-reactivity or interference between Human CGRP1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human CGRP1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human CGRP1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CGRP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CGRP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human CGRP1. You can calculate the concentration of Human CGRP1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human CGRP1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human CGRP1 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 48.15 145.75 349.43 51.54 133.87 361.48
Standard deviation 3.10 8.31 13.66 3.40 6.84 13.59
CV (%) 6.44 5.70 3.91 6.60 5.11 3.76

Recovery

The recovery of Human CGRP1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 89-103 96
EDTA plasma (n=8) 87-98 92
Cell culture media (n=8) 91-105 96

Linearity

Samples were spiked with high concentrations of Human CGRP1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P06881
SynonymsCGRP1
Research AreaMetabolism, Signal transduction, Cancer

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Evidence-based Complementary and Alternative Medicine (2019) IF: 1.984
    The Efficacy of Shinbaro for the Preventive Treatment of Migraine: A Pilot Study

    DOI: 10.1155/2019/2363420

    Sample: Serum
  2. Food & Function (2022) IF: 5.396
    A randomised, double-blind, placebo-controlled trial of Bifidobacterium bifidum CCFM16 for manipulation of gut microbiota and relief from chronic constipation

    DOI: 10.1039/D1FO03896F

    Sample: Serum
  3. Cell Metabolism (2022) IF: 31.373
    Cancer cells co-opt nociceptive nerves to thrive in nutrient-poor environments and upon nutrient-starvation therapies

    DOI: 10.1016/j.cmet.2022.10.012

    PMID: 36395769

    Sample: Plasma
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Reviews/Q&A

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  • Reviews
  • Q&A
Q

J**********dSubmitted [ Oct 13 2023 ]

Asked: Is extraction required for the samples being used with this kit or just dilution?

Reply

A

adminSubmitted [ Oct 13 2023 ]

Answered: Hi Jess, for secretory samples, such as serum/plasma, it is generally recommended not to dilute the test and there are no extraction steps. Serum/plasma samples should be prepared as follows: Serum: Allow samples to clot for 1 hour at room temperature or overnight at 2-8℃beforecentrifugation for 20 min at 1000×g at 2-8℃. Collect the supernatant to carry out the assay. Plasma: Collect plasma using EDTA-Na2 as an anticoagulant. Centrifuge samples for 15min at 1000×g at 2-8℃ within 30 min of collection. Collect the supernatant to carry outthe assay. If you need any other assistance, please let us know. Have a nice day!

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