Human CLDN11 (Claudin 11) CLIA Kit (E-CL-H0591)

Name: Human CLDN11 (Claudin 11) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0591
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human CLDN11 in samples.No significant cross-reactivity or interference between Human CLDN11 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	54689.000	59761.000	57225.000	57184
1000	23736.000	26606.000	25171.000	25130
500	13157.000	10989.000	12073.000	12032
250	5269.000	6051.000	5660.000	5619
125	2960.000	2658.000	2809.000	2768
62.5	1413.000	1407.000	1410.000	1369
31.25	647.000	737.000	692.000	651
0	41.000	41.000	41.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	106.090	299.690	954.310	104.160	329.590	933.230
Standard deviation	11.320	27.330	103.920	12.780	38.330	92.950
CV（%）	10.670	9.120	10.890	12.270	11.630	9.960

Recovery

The recovery of Human CLDN11 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	99-114	107
EDTA plasma （n=8）	89-103	94
Cell culture media （n=8）	87-101	93

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human CLDN11 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	101-120	99-114	100-116
1:2	Average	109	105	107
1:4	Range	101-118	101-117	99-112
1:4	Average	109	107	105
1:8	Range	99-114	90-104	90-101
1:8	Average	106	95	96

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CLDN11. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CLDN11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CLDN11, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human CLDN11. You can calculate the concentration of Human CLDN11 in the samples by comparing the RLU value of the samples to the standard curve.

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