Human CLDN1 (Claudin 1) CLIA Kit (E-CL-H0538)

Name: Human CLDN1 (Claudin 1) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0538
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human CLDN1 in samples.No significant cross-reactivity or interference between Human CLDN1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	57670.000	61392.000	59531.000	59500
1000	23895.000	26017.000	24956.000	24925
500	12333.000	11743.000	12038.000	12007
250	5336.000	5928.000	5632.000	5601
125	2765.000	2633.000	2699.000	2668
62.5	1398.000	1332.000	1365.000	1334
31.25	666.000	714.000	690.000	659
0	30.000	32.000	31.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	100.240	311.520	795.720	92.500	327.000	852.760
Standard deviation	10.530	27.070	77.500	11.730	38.880	57.130
CV（%）	10.500	8.690	9.740	12.680	11.890	6.700

Recovery

The recovery of Human CLDN1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	84-100	91
EDTA plasma （n=8）	97-108	103
Cell culture media （n=8）	96-111	102

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human CLDN1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	94-108	91-105	100-114
1:2	Average	100	96	107
1:4	Range	93-108	92-109	85-98
1:4	Average	100	100	92
1:8	Range	98-111	88-101	95-107
1:8	Average	105	94	102

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CLDN1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CLDN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CLDN1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human CLDN1. You can calculate the concentration of Human CLDN1 in the samples by comparing the RLU value of the samples to the standard curve.

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