Human Collagenase I CLIA Kit (E-CL-H0566)

Name: Human Collagenase I CLIA Kit
Brand: Elabscience
SKU: E-CL-H0566
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human Collagenase I in samples.No significant cross-reactivity or interference between Human Collagenase I and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	57980.000	64200.000	61090.000	61053
1000	24071.000	24537.000	24304.000	24267
500	12912.000	10782.000	11847.000	11810
250	5364.000	5866.000	5615.000	5578
125	2866.000	2444.000	2655.000	2618
62.5	1358.000	1310.000	1334.000	1297
31.25	668.000	682.000	675.000	638
0	37.000	37.000	37.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	91.360	184.940	860.080	83.720	183.610	883.100
Standard deviation	8.670	16.390	83.170	9.640	17.060	59.870
CV（%）	9.490	8.860	9.670	11.510	9.290	6.780

Recovery

The recovery of Human CollagenaseI was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	84-98	90
EDTA plasma （n=8）	86-98	91
Cell culture media （n=8）	86-100	93

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human CollagenaseI and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	101-115	95-111	93-109
1:2	Average	107	103	100
1:4	Range	89-99	100-118	100-117
1:4	Average	94	108	108
1:8	Range	87-99	95-111	100-116
1:8	Average	92	103	106

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Collagenase I. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Collagenase I and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Collagenase I, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human Collagenase I. You can calculate the concentration of Human Collagenase I in the samples by comparing the RLU value of the samples to the standard curve.

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