Human CRLF1 (Cytokine Receptor Like Factor 1) CLIA Kit (E-CL-H0292)

Name: Human CRLF1 (Cytokine Receptor Like Factor 1) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0292
Price: 180.0 USD
Availability: InStock

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Human CRLF1 (Cytokine Receptor Like Factor 1) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human CRLF1 in samples.No significant cross-reactivity or interference between Human CRLF1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	55157.000	66107.000	60632.000	60593
1000	25201.000	25653.000	25427.000	25388
500	11882.000	10788.000	11335.000	11296
250	5538.000	5624.000	5581.000	5542
125	2866.000	2842.000	2854.000	2815
62.5	1433.000	1325.000	1379.000	1340
31.25	681.000	705.000	693.000	654
0	38.000	40.000	39.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	96.090	230.640	674.620	103.180	215.230	707.300
Standard deviation	10.540	18.040	44.790	8.780	19.410	67.480
CV（%）	10.970	7.820	6.640	8.510	9.020	9.540

Recovery

The recovery of Human CRLF1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	86-98	91
EDTA plasma （n=8）	92-105	99
Cell culture media （n=8）	97-111	103

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human CRLF1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	101-116	91-105	89-101
1:2	Average	108	99	95
1:4	Range	101-115	87-98	85-97
1:4	Average	108	92	90
1:8	Range	85-100	99-116	99-114
1:8	Average	91	107	105

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human CRLF1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human CRLF1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human CRLF1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human CRLF1. You can calculate the concentration of Human CRLF1 in the samples by comparing the RLU value of the samples to the standard curve.

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