Human cTn-I/TNNI3 (cardiac Troponin I)CLIA Kit (E-CL-H0495)

Name: Human cTn-I/TNNI3 (cardiac Troponin I)CLIA Kit
Brand: Elabscience
SKU: E-CL-H0495
Price: 180.0 USD
Availability: InStock

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Human cTn-I/TNNI3 (cardiac Troponin I)CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	46.88 pg/mL
Detection Range	78.13-5000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human cTn-I/TNNI3 in samples.No significant cross-reactivity or interference between Human cTn-I/TNNI3 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
5000	58295.000	71145.000	64720.000	64678
2500	34172.000	40586.000	37379.000	37337
1250	20353.000	18581.000	19467.000	19425
625	10241.000	10325.000	10283.000	10241
312.5	5490.000	4894.000	5192.000	5150
156.25	2776.000	2434.000	2605.000	2563
78.13	1338.000	1406.000	1372.000	1330
0	40.000	44.000	42.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	254.530	513.410	1903.300	257.460	557.970	1898.330
Standard deviation	24.890	60.020	156.640	30.050	44.300	184.520
CV（%）	9.780	11.690	8.230	11.670	7.940	9.720

Recovery

The recovery of Human cTn-I was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	100-116	107
EDTA plasma （n=8）	92-106	99
Cell culture media （n=8）	93-107	98

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human cTn-I and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	94-108	101-115	92-104
1:2	Average	100	106	97
1:4	Range	97-111	86-98	95-110
1:4	Average	102	92	101
1:8	Range	86-101	97-113	85-100
1:8	Average	92	105	91

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human cTn-I/TNNI3. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human cTn-I/TNNI3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human cTn-I/TNNI3, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human cTn-I/TNNI3. You can calculate the concentration of Human cTn-I/TNNI3 in the samples by comparing the RLU value of the samples to the standard curve.

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