Human DEFβ2/DEFB2 (Defensin Beta 2) CLIA Kit (E-CL-H0671)

Name: Human DEFβ2/DEFB2 (Defensin Beta 2) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0671
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human DEFβ2/DEFB2 (Defensin Beta 2) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human DEFβ2/DEFB2 in samples.No significant cross-reactivity or interference between Human DEFβ2/DEFB2 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	54109.000	55803.000	54956.000	54925
500	24446.000	24772.000	24609.000	24578
250	11518.000	10068.000	10793.000	10762
125	4875.000	5093.000	4984.000	4953
62.5	2735.000	2555.000	2645.000	2614
31.25	1471.000	1303.000	1387.000	1356
15.63	737.000	761.000	749.000	718
0	30.000	32.000	31.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	47.880	120.010	365.520	47.550	127.940	355.590
Standard deviation	4.770	11.880	25.810	5.120	13.790	32.610
CV（%）	9.960	9.900	7.060	10.770	10.780	9.170

Recovery

The recovery of Human DEFβ2 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	99-117	107
EDTA plasma （n=8）	96-113	103
Cell culture media （n=8）	96-111	103

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human DEFβ2 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	103-119	88-98	94-105
1:2	Average	110	93	99
1:4	Range	93-110	89-102	103-118
1:4	Average	100	94	109
1:8	Range	86-100	98-111	98-113
1:8	Average	92	104	106

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human DEFβ2/DEFB2. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human DEFβ2/DEFB2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human DEFβ2/DEFB2, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human DEFβ2/DEFB2. You can calculate the concentration of Human DEFβ2/DEFB2 in the samples by comparing the RLU value of the samples to the standard curve.

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