Human EPO (Erythropoietin) CLIA Kit (E-CL-H0066)

Name: Human EPO (Erythropoietin) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0066
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	7.5 pg/mL
Detection Range	12.5-800 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human EPO in samples.No significant cross-reactivity or interference between Human EPO and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
800	52182.000	63550.000	57866.000	57833
400	22088.000	22502.000	22295.000	22262
200	10825.000	10493.000	10659.000	10626
100	4483.000	4625.000	4554.000	4521
50	2404.000	2180.000	2292.000	2259
25	1120.000	1060.000	1090.000	1057
12.5	505.000	569.000	537.000	504
0	32.000	34.000	33.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	40.840	104.280	326.110	39.080	94.620	296.040
Standard deviation	4.140	7.360	32.710	3.560	11.070	22.110
CV（%）	10.140	7.060	10.030	9.110	11.700	7.470

Recovery

The recovery of Human EPO was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	88-102	95
EDTA plasma （n=8）	94-107	102
Cell culture media （n=8）	95-111	103

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human EPO and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	98-114	93-110	103-116
1:2	Average	104	100	109
1:4	Range	86-98	87-100	98-115
1:4	Average	91	93	106
1:8	Range	92-106	100-116	97-111
1:8	Average	99	109	103

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human EPO. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human EPO and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human EPO, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human EPO. You can calculate the concentration of Human EPO in the samples by comparing the RLU value of the samples to the standard curve.

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