Human ERα (Estrogen Receptor Alpha) CLIA Kit (E-CL-H0755)

Name: Human ERα (Estrogen Receptor Alpha) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0755
Price: 180.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human ERα (Estrogen Receptor Alpha) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human ERα in samples.No significant cross-reactivity or interference between Human ERα and analogues was observed
Recovery	80%-120%
Sample Type	Tissue homogenates, cell lysates and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	55027.000	60327.000	57677.000	57650
500	23273.000	23781.000	23527.000	23500
250	11049.000	10167.000	10608.000	10581
125	5202.000	5554.000	5378.000	5351
62.5	2666.000	2612.000	2639.000	2612
31.25	1381.000	1299.000	1340.000	1313
15.63	712.000	738.000	725.000	698
0	26.000	28.000	27.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	45.580	84.750	444.050	48.270	81.150	406.260
Standard deviation	5.160	7.440	38.010	5.240	9.220	30.390
CV（%）	11.320	8.780	8.560	10.860	11.360	7.480

Recovery

The recovery of Human ERα was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	94-111	102
EDTA plasma （n=8）	100-112	106
Cell culture media （n=8）	84-95	90

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human ERα and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	89-101	96-112	86-100
1:2	Average	95	103	93
1:4	Range	102-116	96-111	101-119
1:4	Average	108	103	110
1:8	Range	92-109	88-100	87-102
1:8	Average	99	95	93

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ERα. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ERα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ERα, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ERα. You can calculate the concentration of Human ERα in the samples by comparing the RLU value of the samples to the standard curve.

Background