Human ET-1 (Endothelin 1) CLIA Kit
Price: $ 682
Price: $ 546
Price: $ 180
Price: Inquire
Price: Inquire
Size:
96T
48T
24T
96T*10
96T*5
Quantity:
- Reactivity: Human
- Detection Range: 7.81-500 pg/mL
- Sensitivity: 4.69 pg/mL
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Product Details
Test Principle | This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human ET-1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human ET-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ET-1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human ET-1. You can calculate the concentration of Human ET-1 in the samples by comparing the RLU value of the samples to the standard curve. |
Assay Type | Sandwich-ELISA |
size | 96T / 48T / 24T / 96T*10 / 96T*5 |
Assay Time | 3.5h |
Detection Method | Chemiluminescence |
Detection Range | 7.81-500 pg/mL |
Sensitivity | 4.69 pg/mL |
Sample Volume | 100μL |
Sample Type | serum, plasma and other biological fluids |
Specificity | This kit recognizes Human ET-1 in samples.No significant cross-reactivity or interference between Human ET-1 and analogues was observed |
Precision | Both intra-CV and inter-CV are < 10%. |
Recovery | 80%-120% |
Introduction | This ELISA kit applies to the in vitro quantitative determination of Human ET-1 concentrations in serum, plasma and other biological fluids. |
Applications | CLIA |
Storage | 2-8℃/-20℃ |
Target Information
Database | SwissProt: P05305 |
Syonoyms | EDN1,ET1,HDLCQ7,PPET1,Preproendothelin-1 |
Research Area | Cancer , Cardiovascular , Metabolism , Signal Transduction |
Assay Procedures
Citations
-
Metformin alleviates bevacizumab-induced vascular endothelial injury by up-regulating GDF15 and activating the PI3K/AKT/FOXO/PPARγ signaling pathway
IF:3.932
Journal:Annals of Translational Medicine
PMID:34790753
-
AGEs/RAGE blockade downregulates Endothenin-1 (ET-1), mitigating Human Umbilical Vein Endothelial Cells (HUVEC) injury in deep vein thrombosis (DVT)
IF:3.269
Journal:Bioengineered
DOI:10.1080/21655979.2021.1917980
PMID:33896376