Human FABP3 (Fatty Acid Binding Protein 3, Muscle and Heart) CLIA Kit (E-CL-H0919)

Name: Human FABP3 (Fatty Acid Binding Protein 3, Muscle and Heart) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0919
Price: 180.0 USD
Availability: InStock

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Human FABP3 (Fatty Acid Binding Protein 3, Muscle and Heart) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human FABP3 in samples.No significant cross-reactivity or interference between Human FABP3 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	53884.000	63478.000	58681.000	58649
1000	23737.000	27799.000	25768.000	25736
500	12023.000	10591.000	11307.000	11275
250	5443.000	5621.000	5532.000	5500
125	2870.000	2792.000	2831.000	2799
62.5	1410.000	1400.000	1405.000	1373
31.25	636.000	714.000	675.000	643
0	31.000	33.000	32.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	102.640	225.200	728.630	93.930	228.990	730.080
Standard deviation	9.670	15.760	83.570	11.670	23.700	78.630
CV（%）	9.420	7.000	11.470	12.420	10.350	10.770

Recovery

The recovery of Human FABP3 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	97-111	105
EDTA plasma （n=8）	98-115	105
Cell culture media （n=8）	98-113	104

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human FABP3 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	87-100	88-101	90-104
1:2	Average	94	96	96
1:4	Range	88-104	89-104	93-103
1:4	Average	95	96	98
1:8	Range	89-103	97-111	94-110
1:8	Average	94	104	101

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FABP3. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FABP3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FABP3, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human FABP3. You can calculate the concentration of Human FABP3 in the samples by comparing the RLU value of the samples to the standard curve.

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