Human FBN1 (Fibrillin 1) CLIA Kit (E-CL-H1335)

Name: Human FBN1 (Fibrillin 1) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1335
Price: 180.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human FBN1 in samples.No significant cross-reactivity or interference between Human FBN1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	58443.000	69529.000	63986.000	63950
2000	27586.000	31802.000	29694.000	29658
1000	14518.000	13056.000	13787.000	13751
500	6777.000	6807.000	6792.000	6756
250	3633.000	3353.000	3493.000	3457
125	1769.000	1595.000	1682.000	1646
62.5	746.000	832.000	789.000	753
0	35.000	37.000	36.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	205.110	481.990	1963.810	204.030	517.480	2021.960
Standard deviation	23.380	38.080	134.520	19.650	42.280	215.540
CV（%）	11.400	7.900	6.850	9.630	8.170	10.660

Recovery

The recovery of Human FBN1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	100-116	106
EDTA plasma （n=8）	97-109	103
Cell culture media （n=8）	92-103	98

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human FBN1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	94-111	90-103	99-113
1:2	Average	102	98	107
1:4	Range	91-105	93-104	100-118
1:4	Average	97	98	108
1:8	Range	87-99	87-98	99-114
1:8	Average	93	93	105

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FBN1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FBN1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FBN1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human FBN1. You can calculate the concentration of Human FBN1 in the samples by comparing the RLU value of the samples to the standard curve.

Background