Human FE (Ferritin) CLIA Kit (E-CL-H0155)

Name: Human FE (Ferritin) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0155
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human FE in samples.No significant cross-reactivity or interference between Human FE and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	59844.000	61678.000	60761.000	60732
1000	24402.000	25556.000	24979.000	24950
500	12389.000	10687.000	11538.000	11509
250	5593.000	5985.000	5789.000	5760
125	2895.000	2601.000	2748.000	2719
62.5	1412.000	1406.000	1409.000	1380
31.25	683.000	717.000	700.000	671
0	29.000	29.000	29.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	92.680	179.570	890.550	84.340	175.840	910.590
Standard deviation	8.580	18.870	90.750	8.110	17.060	66.750
CV（%）	9.260	10.510	10.190	9.620	9.700	7.330

Recovery

The recovery of Human FE was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	95-109	102
EDTA plasma （n=8）	99-112	106
Cell culture media （n=8）	99-115	106

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human FE and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	98-116	91-101	98-113
1:2	Average	106	96	104
1:4	Range	91-105	97-111	95-108
1:4	Average	98	105	102
1:8	Range	93-106	92-104	99-116
1:8	Average	100	97	106

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FE. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FE, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human FE. You can calculate the concentration of Human FE in the samples by comparing the RLU value of the samples to the standard curve.

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