Human FGF23 (Fibroblast Growth Factor 23) CLIA Kit (E-CL-H0747)

Name: Human FGF23 (Fibroblast Growth Factor 23) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0747
Price: 180.0 USD
Availability: InStock

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Human FGF23 (Fibroblast Growth Factor 23) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	4.69 pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human FGF23 in samples.No significant cross-reactivity or interference between Human FGF23 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
500	50192.000	59446.000	54819.000	54790
250	21721.000	21757.000	21739.000	21710
125	9953.000	9465.000	9709.000	9680
62.5	4783.000	5009.000	4896.000	4867
31.25	2398.000	2280.000	2339.000	2310
15.63	1355.000	1249.000	1302.000	1273
7.81	747.000	769.000	758.000	729
0	28.000	30.000	29.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	26.610	79.410	223.950	25.490	71.560	238.820
Standard deviation	2.250	7.120	23.810	2.410	7.860	26.770
CV（%）	8.460	8.970	10.630	9.450	10.980	11.210

Recovery

The recovery of Human FGF23 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	85-99	91
EDTA plasma （n=8）	91-105	98
Cell culture media （n=8）	86-101	93

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human FGF23 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	91-104	91-105	96-111
1:2	Average	98	98	101
1:4	Range	85-99	98-113	90-103
1:4	Average	90	104	97
1:8	Range	91-103	91-106	89-106
1:8	Average	98	97	97

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FGF23. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGF23 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGF23, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human FGF23. You can calculate the concentration of Human FGF23 in the samples by comparing the RLU value of the samples to the standard curve.

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