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Human FGF23 (Fibroblast Growth Factor 23) CLIA Kit

Cat:E-CL-H0747
Manual MSDS

Price: $ 682

Price: $ 546

Price: $ 180

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Price: Inquire

Size:
96T 48T 24T 96T*10 96T*5
Quantity:
  • Reactivity: Human
  • Detection Range: 7.81-500 pg/mL
  • Sensitivity: 4.69 pg/mL
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Product Details
Test Principle This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FGF23. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGF23 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGF23, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human FGF23. You can calculate the concentration of Human FGF23 in the samples by comparing the RLU value of the samples to the standard curve.
Assay Type Sandwich-ELISA
size 96T / 48T / 24T / 96T*10 / 96T*5
Assay Time 3.5h
Detection Method Chemiluminescence
Detection Range 7.81-500 pg/mL
Sensitivity 4.69 pg/mL
Sample Volume 100μL
Sample Type serum, plasma and other biological fluids
Specificity This kit recognizes Human FGF23 in samples.No significant cross-reactivity or interference between Human FGF23 and analogues was observed
Precision Both intra-CV and inter-CV are < 10%.
Recovery 80%-120%
Introduction This ELISA kit applies to the in vitro quantitative determination of Human FGF23 concentrations in serum, plasma and other biological fluids.
Applications CLIA
Storage 2-8℃/-20℃
Database SwissProt:  Q9GZV9
Syonoyms FGF-23,ADHR,FGFN,HYPF,HPDR2,PHPTC
Research Area Cancer Developmental Biology Signal Transduction Stem Cells