Human FGβ (Fibrinogen Beta) CLIA Kit (E-CL-H1266)

Name: Human FGβ (Fibrinogen Beta) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1266
Price: 180.0 USD
Availability: InStock

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Human FGβ (Fibrinogen Beta) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.19 ng/mL
Detection Range	0.31-20 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human FGβ in samples.No significant cross-reactivity or interference between Human FGβ and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
20	34563.000	39773.000	37168.000	37135
10	12063.000	13893.000	12978.000	12945
5	5850.000	5564.000	5707.000	5674
2.5	2576.000	2958.000	2767.000	2734
1.25	1499.000	1425.000	1462.000	1429
0.63	954.000	834.000	894.000	861
0.31	644.000	708.000	676.000	643
0	32.000	34.000	33.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	0.920	2.570	7.050	0.870	2.550	6.990
Standard deviation	0.090	0.190	0.500	0.100	0.240	0.780
CV（%）	9.780	7.390	7.090	11.490	9.410	11.160

Recovery

The recovery of Human FGβ was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	93-107	101
EDTA plasma （n=8）	88-104	95
Cell culture media （n=8）	94-110	100

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human FGβ and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	96-114	97-114	101-119
1:2	Average	104	104	108
1:4	Range	91-107	88-104	87-103
1:4	Average	98	95	94
1:8	Range	87-102	84-96	93-106
1:8	Average	95	90	98

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FGβ. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FGβ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FGβ, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human FGβ. You can calculate the concentration of Human FGβ in the samples by comparing the RLU value of the samples to the standard curve.

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