Human FMOD (Fibromodulin) CLIA Kit (E-CL-H1258)

Name: Human FMOD (Fibromodulin) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1258
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human FMOD in samples.No significant cross-reactivity or interference between Human FMOD and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	59076.000	60552.000	59814.000	59783
2000	29709.000	29755.000	29732.000	29701
1000	14475.000	13789.000	14132.000	14101
500	6569.000	7443.000	7006.000	6975
250	3318.000	3224.000	3271.000	3240
125	1732.000	1640.000	1686.000	1655
62.5	761.000	813.000	787.000	756
0	31.000	31.000	31.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	188.350	345.030	1674.250	174.750	347.480	1630.150
Standard deviation	19.550	24.500	145.830	21.900	29.120	176.550
CV（%）	10.380	7.100	8.710	12.530	8.380	10.830

Recovery

The recovery of Human FMOD was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	88-99	93
EDTA plasma （n=8）	95-105	100
Cell culture media （n=8）	87-102	93

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human FMOD and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	98-111	93-107	98-111
1:2	Average	104	99	106
1:4	Range	95-111	84-97	95-113
1:4	Average	103	91	103
1:8	Range	94-106	84-97	96-113
1:8	Average	101	91	104

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FMOD. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FMOD and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FMOD, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human FMOD. You can calculate the concentration of Human FMOD in the samples by comparing the RLU value of the samples to the standard curve.

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