Human FN (Fibronectin) CLIA Kit (E-CL-H0163)

Name: Human FN (Fibronectin) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0163
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.19 ng/mL
Detection Range	0.31-20 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human FN in samples.No significant cross-reactivity or interference between Human FN and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
20	34515.000	37623.000	36069.000	36040
10	13219.000	13507.000	13363.000	13334
5	5896.000	5516.000	5706.000	5677
2.5	2493.000	2949.000	2721.000	2692
1.25	1605.000	1387.000	1496.000	1467
0.63	904.000	890.000	897.000	868
0.31	638.000	686.000	662.000	633
0	28.000	30.000	29.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	0.940	2.310	6.820	0.940	2.200	7.170
Standard deviation	0.100	0.220	0.550	0.080	0.250	0.540
CV（%）	10.640	9.520	8.060	8.510	11.360	7.530

Recovery

The recovery of Human FN was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	88-104	95
EDTA plasma （n=8）	96-112	104
Cell culture media （n=8）	95-109	100

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human FN and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	99-113	93-107	96-113
1:2	Average	106	98	103
1:4	Range	89-106	91-104	90-101
1:4	Average	97	97	95
1:8	Range	100-116	100-113	101-118
1:8	Average	107	107	108

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human FN. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human FN and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human FN, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human FN. You can calculate the concentration of Human FN in the samples by comparing the RLU value of the samples to the standard curve.

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