Human GAP43 (Growth Associated Protein 43) CLIA Kit (E-CL-H1188)

Name: Human GAP43 (Growth Associated Protein 43) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1188
Price: 180.0 USD
Availability: InStock

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Human GAP43 (Growth Associated Protein 43) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human GAP43 in samples.No significant cross-reactivity or interference between Human GAP43 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	55367.000	60027.000	57697.000	57664
1000	24464.000	26754.000	25609.000	25576
500	11989.000	10645.000	11317.000	11284
250	5018.000	5984.000	5501.000	5468
125	2848.000	2470.000	2659.000	2626
62.5	1474.000	1400.000	1437.000	1404
31.25	658.000	710.000	684.000	651
0	32.000	34.000	33.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	94.410	287.040	773.360	102.980	274.690	735.860
Standard deviation	7.590	25.030	49.960	10.740	32.470	61.520
CV（%）	8.040	8.720	6.460	10.430	11.820	8.360

Recovery

The recovery of Human GAP43 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	100-112	106
EDTA plasma （n=8）	89-101	96
Cell culture media （n=8）	93-107	101

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human GAP43 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	96-111	102-119	100-116
1:2	Average	102	110	107
1:4	Range	88-100	88-102	96-109
1:4	Average	94	95	102
1:8	Range	98-113	85-97	95-111
1:8	Average	105	91	103

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GAP43. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GAP43 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GAP43, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human GAP43. You can calculate the concentration of Human GAP43 in the samples by comparing the RLU value of the samples to the standard curve.

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