Human GDF11 (Growth Differentiation Factor 11) CLIA Kit (E-CL-H1180)

Name: Human GDF11 (Growth Differentiation Factor 11) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1180
Price: 180.0 USD
Availability: InStock

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Human GDF11 (Growth Differentiation Factor 11) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	4.69 pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human GDF11 in samples.No significant cross-reactivity or interference between Human GDF11 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
500	50168.000	58460.000	54314.000	54284
250	20863.000	23797.000	22330.000	22300
125	10372.000	9736.000	10054.000	10024
62.5	4542.000	4674.000	4608.000	4578
31.25	2517.000	2227.000	2372.000	2342
15.63	1378.000	1192.000	1285.000	1255
7.81	739.000	803.000	771.000	741
0	29.000	31.000	30.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	26.250	45.850	228.710	25.830	41.890	226.120
Standard deviation	2.700	4.650	23.490	2.240	4.710	23.920
CV（%）	10.290	10.140	10.270	8.670	11.240	10.580

Recovery

The recovery of Human GDF11 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	85-99	90
EDTA plasma （n=8）	101-113	107
Cell culture media （n=8）	87-99	93

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human GDF11 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	94-108	95-108	98-112
1:2	Average	102	101	104
1:4	Range	101-118	94-112	98-112
1:4	Average	110	102	104
1:8	Range	88-102	91-105	101-114
1:8	Average	96	98	107

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GDF11. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GDF11 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GDF11, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human GDF11. You can calculate the concentration of Human GDF11 in the samples by comparing the RLU value of the samples to the standard curve.

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