Human GDF15 (Growth Differentiation Factor 15) CLIA Kit (E-CL-H0080)

Name: Human GDF15 (Growth Differentiation Factor 15) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0080
Price: 180.0 USD
Availability: InStock

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Human GDF15 (Growth Differentiation Factor 15) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	4.69 pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human GDF15 in samples.No significant cross-reactivity or interference between Human GDF15 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
500	51777.000	52293.000	52035.000	52005
250	19954.000	23626.000	21790.000	21760
125	9687.000	9555.000	9621.000	9591
62.5	4293.000	4827.000	4560.000	4530
31.25	2492.000	2232.000	2362.000	2332
15.63	1331.000	1183.000	1257.000	1227
7.81	761.000	809.000	785.000	755
0	29.000	31.000	30.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	24.250	74.900	217.760	21.930	74.730	209.400
Standard deviation	2.260	8.800	24.820	2.430	7.880	21.710
CV（%）	9.320	11.750	11.400	11.080	10.540	10.370

Recovery

The recovery of Human GDF15 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	100-112	107
EDTA plasma （n=8）	85-99	92
Cell culture media （n=8）	94-108	99

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human GDF15 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	86-100	89-103	93-106
1:2	Average	94	95	100
1:4	Range	96-113	92-105	96-109
1:4	Average	103	98	102
1:8	Range	96-107	90-107	96-110
1:8	Average	101	98	104

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GDF15. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GDF15 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GDF15, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human GDF15. You can calculate the concentration of Human GDF15 in the samples by comparing the RLU value of the samples to the standard curve.

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