Human GFAP (Glial Fibrillary Acidic Protein) CLIA Kit (E-CL-H1167)

Name: Human GFAP (Glial Fibrillary Acidic Protein) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1167
Price: 180.0 USD
Availability: InStock

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Human GFAP (Glial Fibrillary Acidic Protein) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human GFAP in samples.No significant cross-reactivity or interference between Human GFAP and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	54336.000	65020.000	59678.000	59648
2000	27125.000	32775.000	29950.000	29920
1000	13566.000	13450.000	13508.000	13478
500	6306.000	7418.000	6862.000	6832
250	3612.000	3336.000	3474.000	3444
125	1768.000	1680.000	1724.000	1694
62.5	770.000	868.000	819.000	789
0	29.000	31.000	30.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	216.210	554.750	1858.100	196.810	548.780	1766.950
Standard deviation	23.240	55.250	139.730	19.620	39.900	158.670
CV（%）	10.750	9.960	7.520	9.970	7.270	8.980

Recovery

The recovery of Human GFAP was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	92-106	98
EDTA plasma （n=8）	94-106	101
Cell culture media （n=8）	88-100	93

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human GFAP and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	85-96	89-103	103-120
1:2	Average	91	96	110
1:4	Range	98-110	101-116	97-112
1:4	Average	104	107	104
1:8	Range	98-111	89-102	92-106
1:8	Average	105	96	100

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GFAP. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GFAP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GFAP, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human GFAP. You can calculate the concentration of Human GFAP in the samples by comparing the RLU value of the samples to the standard curve.

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