Human GH2 (Growth Hormone 2) CLIA Kit (E-CL-H1185)

Name: Human GH2 (Growth Hormone 2) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1185
Price: 180.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human GH2 (Growth Hormone 2) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human GH2 in samples.No significant cross-reactivity or interference between Human GH2 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	51450.000	62158.000	56804.000	56773
500	22901.000	23235.000	23068.000	23037
250	11451.000	10285.000	10868.000	10837
125	4803.000	5405.000	5104.000	5073
62.5	2551.000	2539.000	2545.000	2514
31.25	1431.000	1287.000	1359.000	1328
15.63	766.000	776.000	771.000	740
0	30.000	32.000	31.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	48.420	114.820	356.430	49.240	120.040	346.590
Standard deviation	5.690	9.050	21.990	6.350	8.920	22.600
CV（%）	11.750	7.880	6.170	12.900	7.430	6.520

Recovery

The recovery of Human GH2 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	97-114	105
EDTA plasma （n=8）	94-110	102
Cell culture media （n=8）	94-110	100

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human GH2 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	97-110	89-105	98-114
1:2	Average	103	96	106
1:4	Range	101-116	95-108	95-110
1:4	Average	109	100	101
1:8	Range	95-106	85-99	100-112
1:8	Average	100	91	106

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GH2. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GH2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GH2, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human GH2. You can calculate the concentration of Human GH2 in the samples by comparing the RLU value of the samples to the standard curve.

Background