Current Position:Home>>Kits>>ELISA Kits>> Human GHRL(Ghrelin) ELISA Kit

Human GHRL(Ghrelin) ELISA Kit

Citations(12)Uniprot : Q9UBU3

Cat.No.:E-EL-H1919
Reactivity: Human

Filter:

ELISA

To Purchase E-EL-H1919

Size:

96T
48T
24T
96T*5
96T*10

Price: $609

Qty:

Welcome to order from local distributors.

Protocols for ELISA

FAQ for ELISA

Have Questions?

Add to cart Bulk request

For research use only. Order now, ship in 3 days

Product Details

Properties

Assay type	Competitive-ELISA
Format	96T/48T
Assay time	2.0h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 50μL
Specificity	This kit recognizes GHRL in samples. No significant cross-reactivity or interference between GHRL and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of GHRL concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with GHRL. During the reaction, GHRL in the sample or standard competes with a fixed amount of GHRL on the solid phase supporter for sites on the Biotinylated Detection Ab specific to GHRL. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of GHRL in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level GHRL were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level GHRL were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.50	0.90	4.10	0.50	0.90	3.70
Standard deviation	0.03	0.04	0.13	0.03	0.04	0.13
CV (%)	6.00	4.44	3.17	6.00	4.44	3.51

Recovery

The recovery of GHRL spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	86-97	92
EDTA plasma(n=8)	95-108	101
Cell culture media(n=8)	91-105	96

Linearity

Samples were spiked with high concentrations of Human GHRL and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: Q9UBU3
Synonyms	GHRL, MTLRP, Ghrelin
Research Area	Cancer, Cardiovascular, Metabolism, Neuroscience, Signal transduction

Assay Procedures

	1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 45 min at 37°C
	2. Aspirate and wash the plate for 3 times
	3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	5. Add 50μL Stop Solution
	6. Read the plate at 450nm immediately. Calculation of the results

Citations

Frontiers in Nutrition (2022) IF: 6.59
Sleep duration of lactating mothers and its relationship with feeding pattern, milk macronutrients and related serum factors: A combined longitudinal cohort and cross-sectional study

DOI: 10.3389/fnut.2022.973291

PMID: 36110402
Sample: plasma
EUROPEAN JOURNAL OF NUTRITION (2021) IF: 5.619
Effectiveness of a polyphenolic extract (Lippia citriodora and Hibiscus sabdariffa) on appetite regulation in overweight and obese grade I population: an 8-week randomized, double-blind, cross-over, placebo-controlled trial

DOI: 10.1007/s00394-021-02678-x

PMID: 34591168
Sample: blood
OSTEOARTHRITIS AND CARTILAGE (2017) IF: 4.742
Associations between serum ghrelin and knee symptoms, joint structures and cartilage or bone biomarkers in patients with knee osteoarthritis

DOI: 10.1016/j.joca.2017.05.015

Sample: Serum
NEUROPEPTIDES (2021) IF: 3.286
Changes in ghrelin, substance P and vasoactive intestinal peptide levels in the gastroduodenal mucosa of patients with morbid obesity

DOI: 10.1016/j.npep.2021.102164

PMID: 34146741
Sample: serum
International Journal of Environmental Research and Public Health (2021) IF: 3.39
A Case Control Study on Serum Levels of Potential Biomarkers in Male Breast Cancer Patients

DOI: 10.3390/ijerph18094852

PMID: 34062830
Sample: Serum
CYTOKINE (2021) IF: 3.861
The effect of umbilical cord blood spexin, free 25(OH) vitamin D3 and adipocytokine levels on intrauterine growth and anthropometric measurements in newborns

DOI: 10.1016/j.cyto.2021.155578

PMID: 34010726
Sample: Serum
APPETITE (2021) IF: 3.868
Acute and short-term effects of Lactobacillus paracasei subsp. paracasei 431 and inulin intake on appetite control and dietary intake: A two-phases randomized, double blind, placebo-controlled study

DOI: 10.1016/j.appet.2021.105855

PMID: 34915104
Sample: serum
International Journal of Environmental Research and Public Health (2019) IF: 2.468
Effect of Sequence of Fruit Intake in a Meal on Satiety

DOI: 10.3390/ijerph16224464

PMID: 31766283
Sample: Plasma
PSYCHIATRY RESEARCH (2017) IF: 2.528
The association of serum nesfatin-1 and ghrelin levels with metabolic syndrome in patients with schizophrenia

DOI: 10.1016/j.psychres.2017.12.041

Sample: Serum
Biomed Research International (2018) IF: 2.583
High Ghrelin Level Predicts the Curve Progression of Adolescent Idiopathic Scoliosis Girls

DOI: 10.1155/2018/9784083

Sample: Serum
NORDIC JOURNAL OF PSYCHIATRY (2020) IF: 1.78
Serum nesfatin-1, ghrelin, and lipid levels in adolescents with first episode drug naïve unipolar depression

DOI: 10.1080/08039488.2020.1772363

Sample: serum
Journal of Taibah University Medical Sciences (2021)
Does kisspeptin act as a neuropeptide or as an adipokine in obese people?

DOI: 10.1016/j.jtumed.2021.07.010

Sample: serum

Show AllShorten

Reviews/Q&A

Show all
Reviews
Q&A

... Show All Show Less

*Product Name:
*Catalog Number:
Size:
*Name:
*Email:
*Phone:
*Message:
*Country:
*When will you use it?	Within 1 month In 2 to 3 month In 4 to 6 month 6 months later
*Captcha:	Click to load Captcha