Human GRα (Glucocorticoid Receptor Alpha) CLIA Kit (E-CL-H1214)

Name: Human GRα (Glucocorticoid Receptor Alpha) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1214
Price: 180.0 USD
Availability: InStock

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Human GRα (Glucocorticoid Receptor Alpha) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human GRα in samples.No significant cross-reactivity or interference between Human GRα and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	61623.000	63607.000	62615.000	62591
2000	26236.000	31660.000	28948.000	28924
1000	14822.000	12584.000	13703.000	13679
500	6650.000	7240.000	6945.000	6921
250	3299.000	3299.000	3299.000	3275
125	1651.000	1651.000	1651.000	1627
62.5	758.000	804.000	781.000	757
0	23.000	25.000	24.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	192.810	562.620	1478.900	179.910	508.400	1405.650
Standard deviation	21.880	46.130	125.560	16.980	45.650	158.280
CV（%）	11.350	8.200	8.490	9.440	8.980	11.260

Recovery

The recovery of Human GRα was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	98-114	106
EDTA plasma （n=8）	88-101	94
Cell culture media （n=8）	90-102	97

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human GRα and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	91-104	97-111	90-104
1:2	Average	96	103	95
1:4	Range	95-111	96-111	96-112
1:4	Average	101	104	102
1:8	Range	87-100	98-112	84-100
1:8	Average	93	104	91

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GRα. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GRα and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GRα, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human GRα. You can calculate the concentration of Human GRα in the samples by comparing the RLU value of the samples to the standard curve.

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