Human GSTα1 (Glutathione S Transferase Alpha 1) CLIA Kit (E-CL-H0872)

Name: Human GSTα1 (Glutathione S Transferase Alpha 1) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0872
Price: 180.0 USD
Availability: InStock

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Human GSTα1 (Glutathione S Transferase Alpha 1) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.09 ng/mL
Detection Range	0.16-10 ng/mL
Sample Volume	100 μL
Total Assay Time	4 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human GSTα1 in samples.No significant cross-reactivity or interference between Human GSTα1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
10	32794.000	34464.000	33629.000	33598
5	15639.000	17607.000	16623.000	16592
2.5	8259.000	7357.000	7808.000	7777
1.25	3684.000	3828.000	3756.000	3725
0.63	1926.000	1670.000	1798.000	1767
0.31	876.000	804.000	840.000	809
0.16	371.000	415.000	393.000	362
0	30.000	32.000	31.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	0.490	1.500	3.610	0.540	1.440	3.650
Standard deviation	0.060	0.160	0.400	0.050	0.170	0.390
CV（%）	12.240	10.670	11.080	9.260	11.810	10.680

Recovery

The recovery of Human GSTα1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	88-101	95
EDTA plasma （n=8）	86-102	93
Cell culture media （n=8）	85-98	91

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human GSTα1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	94-107	83-96	86-100
1:2	Average	101	90	92
1:4	Range	90-102	99-114	84-99
1:4	Average	96	106	91
1:8	Range	98-112	93-108	89-103
1:8	Average	104	99	94

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GSTα1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GSTα1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GSTα1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human GSTα1. You can calculate the concentration of Human GSTα1 in the samples by comparing the RLU value of the samples to the standard curve.