Human GZMK (Granzyme K) CLIA Kit (E-CL-H0332)

Name: Human GZMK (Granzyme K) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0332
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	7.5 pg/mL
Detection Range	12.5-800 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human GZMK in samples.No significant cross-reactivity or interference between Human GZMK and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
800	49022.000	57934.000	53478.000	53438
400	22147.000	23273.000	22710.000	22670
200	10567.000	9795.000	10181.000	10141
100	4335.000	4895.000	4615.000	4575
50	2472.000	2106.000	2289.000	2249
25	1180.000	1038.000	1109.000	1069
12.5	539.000	543.000	541.000	501
0	39.000	41.000	40.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	42.250	101.750	313.640	38.570	107.210	310.760
Standard deviation	3.630	9.650	28.540	4.990	8.430	34.870
CV（%）	8.590	9.480	9.100	12.940	7.860	11.220

Recovery

The recovery of Human GZMK was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	97-111	105
EDTA plasma （n=8）	99-114	106
Cell culture media （n=8）	94-108	101

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human GZMK and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	85-99	102-119	90-102
1:2	Average	91	109	95
1:4	Range	104-116	95-107	95-109
1:4	Average	110	102	101
1:8	Range	88-101	96-112	97-112
1:8	Average	95	104	103

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human GZMK. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human GZMK and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human GZMK, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human GZMK. You can calculate the concentration of Human GZMK in the samples by comparing the RLU value of the samples to the standard curve.