Human Hepc (Hepcidin) CLIA Kit (E-CL-H0077)

Name: Human Hepc (Hepcidin) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0077
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human Hepc in samples.No significant cross-reactivity or interference between Human Hepc and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	53601.000	63049.000	58325.000	58293
1000	23648.000	25156.000	24402.000	24370
500	12464.000	10854.000	11659.000	11627
250	5606.000	5910.000	5758.000	5726
125	2835.000	2649.000	2742.000	2710
62.5	1331.000	1329.000	1330.000	1298
31.25	710.000	758.000	734.000	702
0	31.000	33.000	32.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	92.270	211.260	844.520	89.920	190.730	828.610
Standard deviation	10.540	20.050	66.380	9.250	14.130	80.710
CV（%）	11.420	9.490	7.860	10.290	7.410	9.740

Recovery

The recovery of Human Hepc was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	90-106	97
EDTA plasma （n=8）	97-111	104
Cell culture media （n=8）	99-114	107

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human Hepc and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	99-112	86-100	101-114
1:2	Average	105	91	108
1:4	Range	100-116	99-115	95-110
1:4	Average	108	106	101
1:8	Range	96-110	100-114	86-100
1:8	Average	101	108	91

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human Hepc. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human Hepc and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human Hepc, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human Hepc. You can calculate the concentration of Human Hepc in the samples by comparing the RLU value of the samples to the standard curve.

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