Human HSP-40 (Heat Shock Protein 40) CLIA Kit (E-CL-H1151)

Name: Human HSP-40 (Heat Shock Protein 40) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1151
Price: 180.0 USD
Availability: InStock

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Human HSP-40 (Heat Shock Protein 40) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human HSP-40 in samples.No significant cross-reactivity or interference between Human HSP-40 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	59650.000	60210.000	59930.000	59899
1000	23295.000	26885.000	25090.000	25059
500	12253.000	11711.000	11982.000	11951
250	5221.000	5831.000	5526.000	5495
125	2891.000	2841.000	2866.000	2835
62.5	1430.000	1288.000	1359.000	1328
31.25	691.000	741.000	716.000	685
0	30.000	32.000	31.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	104.100	176.930	877.450	99.930	193.570	797.780
Standard deviation	9.470	14.900	83.800	12.280	23.170	48.510
CV（%）	9.100	8.420	9.550	12.290	11.970	6.080

Recovery

The recovery of Human HSP-40 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	93-106	100
EDTA plasma （n=8）	85-99	92
Cell culture media （n=8）	92-104	98

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human HSP-40 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	92-106	93-106	99-117
1:2	Average	97	98	108
1:4	Range	101-119	91-102	92-109
1:4	Average	109	96	100
1:8	Range	94-105	103-118	97-111
1:8	Average	100	109	103

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human HSP-40. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human HSP-40 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human HSP-40, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human HSP-40. You can calculate the concentration of Human HSP-40 in the samples by comparing the RLU value of the samples to the standard curve.

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