Human IgA (Immunoglobulin A) CLIA Kit (E-CL-H0892)

Name: Human IgA (Immunoglobulin A) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0892
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human IgA (Immunoglobulin A) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	1.88 ng/mL
Detection Range	3.13-200 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human IgA in samples.No significant cross-reactivity or interference between Human IgA and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
200	43911.000	50139.000	47025.000	46993
100	18075.000	21145.000	19610.000	19578
50	8592.000	8454.000	8523.000	8491
25	3912.000	4344.000	4128.000	4096
12.5	2118.000	2114.000	2116.000	2084
6.25	1169.000	1051.000	1110.000	1078
3.13	614.000	664.000	639.000	607
0	31.000	33.000	32.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	10.120	19.480	72.220	10.380	19.630	70.310
Standard deviation	1.010	2.210	6.460	1.180	1.540	7.470
CV（%）	9.980	11.340	8.940	11.370	7.850	10.620

Recovery

The recovery of Human IgA was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	86-98	91
EDTA plasma （n=8）	90-102	97
Cell culture media （n=8）	92-104	97

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human IgA and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	85-96	91-107	90-102
1:2	Average	91	98	97
1:4	Range	99-111	102-115	84-97
1:4	Average	105	109	91
1:8	Range	87-97	89-105	95-109
1:8	Average	92	96	103

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IgA. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IgA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IgA, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human IgA. You can calculate the concentration of Human IgA in the samples by comparing the RLU value of the samples to the standard curve.

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