Human IgG3 (Immunoglobulin G3) CLIA Kit (E-CL-H1094)

Name: Human IgG3 (Immunoglobulin G3) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1094
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human IgG3 (Immunoglobulin G3) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human IgG3 in samples.No significant cross-reactivity or interference between Human IgG3 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	55895.000	66327.000	61111.000	61082
1000	23863.000	24141.000	24002.000	23973
500	12178.000	11816.000	11997.000	11968
250	5485.000	5729.000	5607.000	5578
125	2982.000	2634.000	2808.000	2779
62.5	1498.000	1286.000	1392.000	1363
31.25	670.000	762.000	716.000	687
0	28.000	30.000	29.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	94.630	243.990	961.690	97.630	229.600	1033.910
Standard deviation	11.550	20.350	105.310	12.510	24.200	90.360
CV（%）	12.210	8.340	10.950	12.810	10.540	8.740

Recovery

The recovery of Human IgG3 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	98-111	104
EDTA plasma （n=8）	93-105	98
Cell culture media （n=8）	100-116	107

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human IgG3 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	95-109	95-108	88-100
1:2	Average	102	100	94
1:4	Range	92-105	94-109	99-117
1:4	Average	100	101	107
1:8	Range	95-110	102-118	101-117
1:8	Average	101	108	107

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IgG3. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IgG3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IgG3, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human IgG3. You can calculate the concentration of Human IgG3 in the samples by comparing the RLU value of the samples to the standard curve.

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