Human IgG4 (Immunoglobulin G4) CLIA Kit (E-CL-H1095)

Name: Human IgG4 (Immunoglobulin G4) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1095
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human IgG4 (Immunoglobulin G4) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.94 ng/mL
Detection Range	1.56-100 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human IgG4 in samples.No significant cross-reactivity or interference between Human IgG4 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
100	34767.000	42441.000	38604.000	38572
50	16903.000	18285.000	17594.000	17562
25	7995.000	7535.000	7765.000	7733
12.5	3641.000	3967.000	3804.000	3772
6.25	1919.000	1881.000	1900.000	1868
3.13	1108.000	1022.000	1065.000	1033
1.56	560.000	596.000	578.000	546
0	31.000	33.000	32.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	4.780	15.360	47.580	4.910	16.800	43.100
Standard deviation	0.400	1.420	2.940	0.630	1.950	3.830
CV（%）	8.370	9.240	6.180	12.830	11.610	8.890

Recovery

The recovery of Human IgG4 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	95-106	101
EDTA plasma （n=8）	85-97	90
Cell culture media （n=8）	83-97	90

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human IgG4 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	100-114	99-113	97-114
1:2	Average	108	106	104
1:4	Range	99-117	90-103	92-109
1:4	Average	107	96	100
1:8	Range	90-106	96-110	94-106
1:8	Average	97	104	101

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IgG4. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IgG4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IgG4, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human IgG4. You can calculate the concentration of Human IgG4 in the samples by comparing the RLU value of the samples to the standard curve.

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