Human IL-10 (Interleukin 10) CLIA Kit (E-CL-H0102)

Name: Human IL-10 (Interleukin 10) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0102
Price: 180.0 USD
Availability: InStock

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Human IL-10 (Interleukin 10) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	4.69 pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human IL-10 in samples.No significant cross-reactivity or interference between Human IL-10 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
500	55009.000	56729.000	55869.000	55834
250	20242.000	23952.000	22097.000	22062
125	9961.000	9427.000	9694.000	9659
62.5	4587.000	5263.000	4925.000	4890
31.25	2638.000	2278.000	2458.000	2423
15.63	1342.000	1332.000	1337.000	1302
7.81	779.000	781.000	780.000	745
0	35.000	35.000	35.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	24.450	46.940	167.570	22.040	49.290	171.490
Standard deviation	3.130	5.510	16.120	2.520	5.520	13.310
CV（%）	12.800	11.740	9.620	11.430	11.200	7.760

Recovery

The recovery of Human IL-10 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	92-106	98
EDTA plasma （n=8）	90-101	95
Cell culture media （n=8）	86-97	91

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human IL-10 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	83-96	99-114	91-105
1:2	Average	90	105	96
1:4	Range	91-106	97-113	102-119
1:4	Average	98	105	109
1:8	Range	94-106	99-116	87-99
1:8	Average	100	106	93

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IL-10. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IL-10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IL-10, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human IL-10. You can calculate the concentration of Human IL-10 in the samples by comparing the RLU value of the samples to the standard curve.

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