Human INSR (Insulin Receptor) CLIA Kit (E-CL-H0368)

Name: Human INSR (Insulin Receptor) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0368
Price: 180.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Human INSR (Insulin Receptor) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	0.19 ng/mL
Detection Range	0.31-20 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human INSR in samples.No significant cross-reactivity or interference between Human INSR and analogues was observed
Recovery	80%-120%
Sample Type	Tissue homogenates, cell lysates and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
20	32805.000	39409.000	36107.000	36076
10	12265.000	13117.000	12691.000	12660
5	5438.000	5260.000	5349.000	5318
2.5	2426.000	2670.000	2548.000	2517
1.25	1461.000	1325.000	1393.000	1362
0.63	1001.000	907.000	954.000	923
0.31	655.000	669.000	662.000	631
0	31.000	31.000	31.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	0.970	2.200	8.260	1.050	2.250	7.930
Standard deviation	0.120	0.200	0.810	0.110	0.200	0.640
CV（%）	12.370	9.090	9.810	10.480	8.890	8.070

Recovery

The recovery of Human INSR was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	100-117	107
EDTA plasma （n=8）	96-113	103
Cell culture media （n=8）	98-116	106

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human INSR and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	87-102	102-113	99-112
1:2	Average	94	108	105
1:4	Range	100-113	96-110	93-105
1:4	Average	106	104	98
1:8	Range	85-97	103-120	88-102
1:8	Average	90	109	94

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human INSR. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human INSR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human INSR, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human INSR. You can calculate the concentration of Human INSR in the samples by comparing the RLU value of the samples to the standard curve.

Background