Human IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa) CLIA Kit (E-CL-H0050)

Name: Human IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0050
Price: 180.0 USD
Availability: InStock

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Human IP-10/CXCL10 (Interferon Gamma Induced Protein 10kDa) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	4.69 pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human IP-10/CXCL10 in samples.No significant cross-reactivity or interference between Human IP-10/CXCL10 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
500	51253.000	61557.000	56405.000	56375
250	21024.000	25002.000	23013.000	22983
125	10079.000	9509.000	9794.000	9764
62.5	4764.000	5126.000	4945.000	4915
31.25	2389.000	2277.000	2333.000	2303
15.63	1380.000	1330.000	1355.000	1325
7.81	742.000	820.000	781.000	751
0	29.000	31.000	30.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	24.600	72.260	192.190	24.270	73.230	175.930
Standard deviation	2.010	6.560	15.340	2.600	8.410	17.430
CV（%）	8.170	9.080	7.980	10.710	11.480	9.910

Recovery

The recovery of Human IP-10 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	94-111	102
EDTA plasma （n=8）	100-115	107
Cell culture media （n=8）	85-96	91

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human IP-10 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	102-118	92-105	93-106
1:2	Average	109	98	98
1:4	Range	98-115	101-116	84-96
1:4	Average	105	109	91
1:8	Range	97-111	87-101	96-110
1:8	Average	104	94	103

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human IP-10/CXCL10. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human IP-10/CXCL10 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human IP-10/CXCL10, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human IP-10/CXCL10. You can calculate the concentration of Human IP-10/CXCL10 in the samples by comparing the RLU value of the samples to the standard curve.

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