Human LDHA (Lactate Dehydrogenase A) CLIA Kit (E-CL-H0438)

Name: Human LDHA (Lactate Dehydrogenase A) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0438
Price: 180.0 USD
Availability: InStock

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Human LDHA (Lactate Dehydrogenase A) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.09 ng/mL
Detection Range	0.16-10 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human LDHA in samples.No significant cross-reactivity or interference between Human LDHA and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
10	32207.000	32841.000	32524.000	32501
5	16325.000	16901.000	16613.000	16590
2.5	7750.000	7366.000	7558.000	7535
1.25	3917.000	4027.000	3972.000	3949
0.63	1811.000	1713.000	1762.000	1739
0.31	916.000	874.000	895.000	872
0.16	383.000	391.000	387.000	364
0	22.000	24.000	23.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	0.460	1.300	4.760	0.460	1.240	5.180
Standard deviation	0.050	0.090	0.500	0.060	0.100	0.540
CV（%）	10.870	6.920	10.500	13.040	8.060	10.420

Recovery

The recovery of Human LDHA was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	91-102	96
EDTA plasma （n=8）	97-111	105
Cell culture media （n=8）	92-106	99

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human LDHA and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	91-105	102-119	99-118
1:2	Average	98	108	108
1:4	Range	99-113	96-112	93-108
1:4	Average	104	104	99
1:8	Range	98-113	102-116	88-103
1:8	Average	104	109	96

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human LDHA. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human LDHA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human LDHA, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human LDHA. You can calculate the concentration of Human LDHA in the samples by comparing the RLU value of the samples to the standard curve.

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