Human MAP-2 (Microtubule Associated Protein 2) CLIA Kit (E-CL-H1231)

Name: Human MAP-2 (Microtubule Associated Protein 2) CLIA Kit
Brand: Elabscience
SKU: E-CL-H1231
Price: 180.0 USD
Availability: InStock

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Human MAP-2 (Microtubule Associated Protein 2) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human MAP-2 in samples.No significant cross-reactivity or interference between Human MAP-2 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	56092.000	67618.000	61855.000	61822
2000	26712.000	30716.000	28714.000	28681
1000	15280.000	13878.000	14579.000	14546
500	6513.000	7379.000	6946.000	6913
250	3764.000	3304.000	3534.000	3501
125	1667.000	1629.000	1648.000	1615
62.5	802.000	808.000	805.000	772
0	32.000	34.000	33.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	189.750	558.350	1717.730	181.580	577.200	1888.360
Standard deviation	16.380	41.490	125.220	22.240	67.880	200.920
CV（%）	8.630	7.430	7.290	12.250	11.760	10.640

Recovery

The recovery of Human MAP-2 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	95-111	103
EDTA plasma （n=8）	92-102	97
Cell culture media （n=8）	84-99	91

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human MAP-2 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	90-105	83-99	88-102
1:2	Average	96	90	96
1:4	Range	100-111	93-108	90-101
1:4	Average	105	99	95
1:8	Range	98-109	101-116	88-100
1:8	Average	103	109	93

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MAP-2. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MAP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MAP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human MAP-2. You can calculate the concentration of Human MAP-2 in the samples by comparing the RLU value of the samples to the standard curve.

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