Human MBP (Myelin Basic Protein) CLIA Kit (E-CL-H0151)

Name: Human MBP (Myelin Basic Protein) CLIA Kit
Brand: Elabscience
SKU: E-CL-H0151
Price: 180.0 USD
Availability: InStock

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Human MBP (Myelin Basic Protein) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	4.69 pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human MBP in samples.No significant cross-reactivity or interference between Human MBP and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
500	47949.000	55953.000	51951.000	51922
250	21996.000	22992.000	22494.000	22465
125	10696.000	9766.000	10231.000	10202
62.5	4527.000	4813.000	4670.000	4641
31.25	2380.000	2276.000	2328.000	2299
15.63	1366.000	1334.000	1350.000	1321
7.81	749.000	823.000	786.000	757
0	28.000	30.000	29.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	26.380	73.460	184.790	23.960	70.980	187.740
Standard deviation	3.230	7.400	11.960	2.670	7.610	21.060
CV（%）	12.240	10.070	6.470	11.140	10.720	11.220

Recovery

The recovery of Human MBP was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	98-112	105
EDTA plasma （n=8）	93-107	100
Cell culture media （n=8）	89-102	94

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human MBP and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	98-112	93-107	92-108
1:2	Average	105	100	99
1:4	Range	95-108	95-111	96-112
1:4	Average	101	103	103
1:8	Range	97-110	101-117	90-100
1:8	Average	105	109	95

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human MBP. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MBP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MBP, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Human MBP. You can calculate the concentration of Human MBP in the samples by comparing the RLU value of the samples to the standard curve.

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