Human MCP-4/CCL13(Monocyte Chemotactic Protein 4) ELISA Kit (E-EL-H1159)

Name: Human MCP-4/CCL13(Monocyte Chemotactic Protein 4) ELISA Kit
Brand: Elabscience
SKU: E-EL-H1159
Price: 150.0 USD
Availability: InStock

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Human MCP-4/CCL13(Monocyte Chemotactic Protein 4) ELISA Kit - 1

Size	Price	Qty
96T	$ 609.00	- +
48T	$ 487.00	- +
24T	$ 150.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Human
Specificity	This kit recognizes Human MCP-4/CCL13 in samples. No significant cross-reactivity or interference between Human MCP-4/CCL13 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	2.469	2.479	2.474	2.401
500	1.597	1.627	1.612	1.539
250	0.982	0.956	0.969	0.896
125	0.470	0.494	0.482	0.409
62.5	0.250	0.236	0.243	0.170
31.25	0.178	0.170	0.174	0.101
15.63	0.120	0.130	0.125	0.052
0	0.065	0.081	0.073	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	46.200	105.530	360.260	42.550	104.550	352.470
Standard deviation	2.590	4.300	10.950	2.460	5.980	10.610
CV（%）	5.610	4.070	3.040	5.780	5.720	3.010

Recovery

The recovery of Human MCP-4 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Cell culture media （n=8）	84-98	90
Serum （n=8）	94-107	99
EDTA plasma （n=8）	85-98	92

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Human MCP-4 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	85-97	95-111	91-105
1:2	Average	90	103	97
1:4	Range	86-97	82-95	89-103
1:4	Average	91	87	95
1:8	Range	82-96	85-99	89-103
1:8	Average	88	92	96

Stability

Each kit batch is subjected to accelerated stability testing and real-time stability monitoring. Sample performance is evaluated after storage at 37 °C for 10 days to assess the impact of elevated temperature on assay reliability and reagent integrity.

/	Variation range of 37°C mean concentration / 2-8°C mean
Sample 1（n=16）	91.43-111.53
Sample 2（n=16）	89.5-92.34

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MCP-4/CCL13. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MCP-4/CCL13 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MCP-4/CCL13, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MCP-4/CCL13. You can calculate the concentration of Human MCP-4/CCL13 in the samples by comparing the OD of the samples to the standard curve.

Background

CCL13 (MCP-4) was initially identified in a library constructed from human fetal mRNA.CCL13 is constitutively expressed at high levels in the small intestine, colon, and lung. Original data showed that CCL13 was less effective than MCP-1 and MCP-3 in monocyte and lymphocyte chemoattraction, but equivalent to eotaxin as a chemoattractant for eosinophils. Chemokines are very important in the inflammatory process, initially in the influx of PMN by CXC chemokines and subsequently chemoattracting monocytes where the MCPs participate, including CCL13. The bioactivity of pro-inflammatory chemokines is regulated by MMPs in order to regulate the influx of different cell subpopulations. CCL13 is involved in inflammatory diseases such as rheumatoid arthritis (RA), atherosclerosis, and asthma. High levels of CCL13 have been detected in the synovial fluid of patients with RA, in serum of asthmatic patients, and in plasma of patients with symptomatic carotid atherosclerosis.