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Human MMP-2(Matrix Metalloproteinase 2) ELISA Kit

  • Cat.No.:E-EL-H1445

  • Reactivity: Human

To Purchase E-EL-H1445

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.78-50 ng/mL
Sensitivity 0.47 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Human MMP-2 in samples. No significant cross-reactivity or interference between Human MMP-2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Human MMP-2 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MMP-2. You can calculate the concentration of Human MMP-2 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human MMP-2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human MMP-2 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 2.40 5.30 21.80 2.40 5.10 20.80
Standard deviation 0.10 0.30 0.90 0.20 0.20 0.70
CV (%) 4.17 5.66 4.13 8.33 3.92 3.37


The recovery of Human MMP-2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-100 92
EDTA plasma (n=8) 87-99 92
Cell culture media (n=8) 88-100 93


Samples were spiked with high concentrations of Human MMP-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database LinksSwissProt:   P08253
SynonymsMMP2, CLG4, CLG4A, MMP-II, MONA, TBE-1, Gelatinase A
Research AreaCancer, Cell Biology, Cardiovascular, Metabolism

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. JOURNAL OF HEPATOLOGY (2019) IF: 18.946
    MMP2/MMP9-mediated CD100 shedding is crucial for inducing intrahepatic anti-HBV CD8 T cell responses and HBV clearance

    DOI: 10.1016/j.jhep.2019.05.013

    PMID: 31173811

    Sample: Serum
  2. Nature Communications (2021) IF: 14.919
    Geometrically encoded SERS nanobarcodes for the logical detection of nasopharyngeal carcinoma-related progression biomarkers

    DOI: 10.1038/s41467-021-23789-3

    PMID: 34078895

    Sample: plasma
    Stiff substrates inhibit collagen accumulation via integrin α2β1, FAK and Src kinases in human atrial fibroblast and myofibroblast cultures derived from patients with aortal stenosis

    DOI: 10.1016/j.biopha.2023.114289

    Sample: cardiac fibroblast
  4. Marine Drugs (2023) IF: 6.085
    Mechanisms of Antitumor Invasion and Metastasis of the Marine Fungal Derivative Epi-Aszonalenin A in HT1080 Cells

    DOI: 10.3390/md21030156

    Sample: HT1080 cell
  5. Cells (2021) IF: 6.6
    The Stiffness of Cardiac Fibroblast Substrates Exerts a Regulatory Influence on Collagen Metabolism via α2β1 Integrin, FAK and Src Kinases

    DOI: 10.3390/cells10123506

    Sample: cardiac fibroblast
    Preparation and evaluation of photoprotective kenaf seed oil nanocarriers-based cream of tocotrienol-rich fraction

    DOI: 10.1016/j.indcrop.2022.115164

    Sample: Normal human dermal fibroblasts (NHDF)
    Downregulation of FoxM1 inhibits proliferation, invasion and angiogenesis of HeLa cells in vitro and in vivo

    DOI: 10.3892/ijo.2014.2645

    Sample: Cell culture supernatant
  8. Inorganic Chemistry Frontiers (2017) IF: 4.036
    Anthraquinone-bridged diruthenium(II) complexes inhibit migration and invasion of human hepatocarcinoma MHCC97-H cells

    DOI: 10.1039/C7QI00149E

    Sample: Cell culture supernatant
  9. Spine Journal (2022) IF: 4.166
    Extracellular vesicle and soluble fractions of adipose tissue-derived mesenchymal stem cells secretome induce inflammatory cytokines modulation in an in vitro model of discogenic pain

    DOI: 10.1016/j.spinee.2022.01.012

    PMID: 35121152

    Sample: human adipose-derived mesenchymal stem cells (HAdM
    Anti-proliferation and anti-inflammation effects of corilagin in rheumatoid arthritis by downregulating NF-κB and MAPK signaling pathways

    DOI: 10.1016/j.jep.2021.114791

    PMID: 34737112

    Sample: MH7A cell
  11. Biomolecules (2019) IF: 4.694
    MMP2 Polymorphism Affects Plasma Matrix Metalloproteinase (MMP)-2 Levels, and Correlates with the Decline in Lung Function in Hypersensitivity Pneumonitis Positive to Autoantibodies Patients

    DOI: 10.3390/biom9100574

    Sample: plasma
  12. MOLECULES (2019) IF: 3.06
    Ampelopsin E Reduces the Invasiveness of the Triple Negative Breast Cancer Cell Line, MDA-MB-231

    DOI: 10.3390/molecules24142619

    Sample: MDA-MB-231 cells
  13. PLACENTA (2022) IF: 3.481
    Fenofibrate mediated activation of PPARα negatively regulates trophoblast invasion

    DOI: 10.1016/j.placenta.2022.05.015

    PMID: 35803128

    Sample: HTR8/SVneo cell
    Serum fibrosis markers could aid in the prediction of factor for successful oral antiviral treatment in hepatitis C

    DOI: 10.1097/MEG.0000000000002083

    PMID: 33560684

    Sample: Blood
    Correlation between proteolytic activity and abdominal aortic aneurysm wall morphology with intraluminal thrombus volume

    DOI: 10.1016/j.avsg.2022.05.039

    PMID: 35779804

    Sample: Abdominal Aortic Aneurysm (AAA) Wall
  16. BIOLOGIA (2022) IF: 1.653
    UPLC-ESI-QTOF-MS/MS and 1H-NMR identification of alkaloids in potent fraction of Catharanthus roseus leaves inhibits migration and invasion of MDA-MB-231 cells

    DOI: 10.1007/s11756-022-01185-6

    Sample: MDA-MB-231 cell
    Expression and Significance of MMPs in Synovial Fluid, Serum and PBMC Culture Supernatant Stimulated by LPS in Osteoarthritis Patients With or Without Diabetes

    DOI: 10.1055/s-0043-122223

    PMID: 29270923

    Sample: Serum,Synovia
  18. BIOLOGIA (2020) IF: 0.8110
    Catharanthus roseus L. extract downregulates the expression profile of motility-related genes in highly invasive human breast cancer cell line MDA-MB-231

    DOI: 10.2478/s11756-020-00641-5

    Sample: MDA-MB-231 cell
    Clinical value of 2D speckle tracking imaging in evaluating the effect of percutaneous intramyocardial septal radiofrequency ablation in patients with hypertrophic obstructive cardiomyopathy

    DOI: 10.1002/jcu.22989

    PMID: 33675543

    Sample: Plasma
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