Human MMP-2(Matrix Metalloproteinase 2) ELISA Kit
MMP2、CLG4、CLG4A、MMP-II、MONA、TBE-1、Gelatinase A
Price: $ 495
Price: $ 396
Price: $ 150
Price: Inquire
Price: Inquire
- Reactivity: Human
- Detection Range: 0.78-50 ng/mL
- Sensitivity: 0.47 ng/mL
For research use only. Order now, ship in 3 days
Test Principle | This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human MMP-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human MMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human MMP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human MMP-2. You can calculate the concentration of Human MMP-2 in the samples by comparing the OD of the samples to the standard curve. |
Assay Type | Sandwich-ELISA |
size | 96T / 48T / 24T / 96T*10 / 96T*5 |
Assay Time | 3.5h |
Detection Method | Colormetric |
Detection Range | 0.78-50 ng/mL |
Sensitivity | 0.47 ng/mL |
Sample Volume | 100μL |
Sample Type | serum, plasma and other biological fluids |
Specificity | This kit recognizes Human MMP-2 in samples. No significant cross-reactivity or interference between Human MMP-2 and analogues was observed |
Precision | Both intra-CV and inter-CV are < 10%. |
Recovery | 80%-120% |
Introduction | This ELISA kit applies to the in vitro quantitative determination of Human MMP-2 concentrations in serum, plasma and other biological fluids. |
Applications | ELISA |
Storage | 2-8℃/-20℃ |
Database | SwissProt: P08253 |
Syonoyms | MMP2,CLG4,CLG4A,MMP-II,MONA,TBE-1,Gelatinase A |
Research Area | Cancer , Cell Biology , Cardiovascular , Metabolism |
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MMP2/MMP9-mediated CD100 shedding is crucial for inducing intrahepatic anti-HBV CD8 T cell responses and HBV clearance
IF:18.946
Journal:JOURNAL OF HEPATOLOGY
DOI:10.1016/j.jhep.2019.05.013
PMID:31173811
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Geometrically encoded SERS nanobarcodes for the logical detection of nasopharyngeal carcinoma-related progression biomarkers
IF:14.919
Journal:Nature Communications
DOI:10.1038/s41467-021-23789-3
PMID:34078895
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Stiff substrates inhibit collagen accumulation via integrin α2β1, FAK and Src kinases in human atrial fibroblast and myofibroblast cultures derived from patients with aortal stenosis
IF:7.419
Journal:BIOMEDICINE & PHARMACOTHERAPY
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The Stiffness of Cardiac Fibroblast Substrates Exerts a Regulatory Influence on Collagen Metabolism via α2β1 Integrin, FAK and Src Kinases
IF:6.6
Journal:Cells
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Mechanisms of Antitumor Invasion and Metastasis of the Marine Fungal Derivative Epi-Aszonalenin A in HT1080 Cells
IF:6.085
Journal:Marine Drugs
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Downregulation of FoxM1 inhibits proliferation, invasion and angiogenesis of HeLa cells in vitro and in vivo
IF:5.65
Journal:INTERNATIONAL JOURNAL OF ONCOLOGY
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Preparation and evaluation of photoprotective kenaf seed oil nanocarriers-based cream of tocotrienol-rich fraction
IF:5.645
Journal:INDUSTRIAL CROPS AND PRODUCTS
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MMP2 Polymorphism Affects Plasma Matrix Metalloproteinase (MMP)-2 Levels, and Correlates with the Decline in Lung Function in Hypersensitivity Pneumonitis Positive to Autoantibodies Patients
IF:4.694
Journal:Biomolecules
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Anti-proliferation and anti-inflammation effects of corilagin in rheumatoid arthritis by downregulating NF-κB and MAPK signaling pathways
IF:4.36
Journal:JOURNAL OF ETHNOPHARMACOLOGY
PMID:34737112
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Extracellular vesicle and soluble fractions of adipose tissue-derived mesenchymal stem cells secretome induce inflammatory cytokines modulation in an in vitro model of discogenic pain
IF:4.166
Journal:Spine Journal
DOI:10.1016/j.spinee.2022.01.012
PMID:35121152
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Q1:Can ELISA measure the cell supernatant MMP-2 and MMP-9? I read the papers written by others, in addition to measuring the expression of these two indicators, but also to measure the activity, can ELISA measure the activity? What's the difference with gelatin enzymology?
These two kits can be used for the detection of your cell supernatant. However, ELISA detection of cell supernatants is affected by factors such as media components, cell growth, and external drug stimulation conditions, and it is recommended that you conduct a preliminary experiment before the formal experiment. Gelatin enzyme spectrometry uses the reversible combination of SDS and MMPs in the sample to separate MMP-2 and MMP-9 in the sample, and then restores the activity of both through the bivalent metal ion buffer system. This test method is specifically aimed at the activity detection of MMP-2 and MMP-9. ELISA detects the content (that is, concentration) of the tested substance through the binding reaction of antigen and antibody, that is, captures the MMP-2 and MMP-9 of the sample through the specific antibodies of MMP-2 and MMP-9. The experimental principles of the two are different, and the activity of MMP-2 and MMP-9 cannot be detected by ELISA.
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Q2:How to operate the "repeated freeze-thaw" process mentioned in tissue or cell sample processing?
Tissue sample: After finishing tissue grinding, place it at -80℃ for 1h/ liquid nitrogen for 0.5h, and then gently shake it in a water bath at 30℃ to melt it quickly. Repeat this operation 1-2 times. Cell sample: Repeat the above freeze-thaw operation 2-3 times. If it is a membrane protein, it can be appropriately sonicated, but the temperature and frequency of ultrasound need to be controlled. It is recommended to add protease inhibitors to the sample in advance. PMSF(Cat.E-EL-SR002) is generally recommended.
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Q3:Can the standard curve in the manual be used directly for calculation?
The standard curve on the manual is an indicator curve, mainly used to show the shape of the standard curve after fitting and the quality control range of the highest/lowest OD value, you can not directly use. In addition, due to the influence of experimental operation, experimental environment, instrument parameter setting and other factors, the OD value of your actual standard curve may not be completely consistent with the instructions (overall high or low). In this case, it is recommended to control the first 4 blue wells in the color development stage with obvious color gradient and the maximum OD value of the standard curve above 1.2. If the correlation coefficient of the standard tune is above 0.99, it can be used as an effective standard curve.
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Q4:The sample/reagent volume is not enough, can I reduce the sample volume uniformly?
Neither can be reduced. The sample volume of each step should be strictly in accordance with the instructions, otherwise the experimental system will be changed, and the results will be inaccurate. If the customer really does not have enough sample volume, you can consult the technology to confirm whether the sample can be diluted, and judge the sample concentration through pre-experiment Degree situation. If the volume of the reagent is not enough, the number of holes to be tested can only be reduced according to the actual volume, and the detection can not be diluted.
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Q5:How long was the ELISA test?
Traditional Competion Method: about 2.0 h; Traditiona three-steps Sandwich method: about 3.5h; QuicKey ELISA: about 2.5h; QuicKey Pro ELISA: about 1.5h.
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Q6:Does ELISA require technical replication? How to set the positive or negative control?
It is recommended that customers do technical repetition, on the one hand can confirm the operation method, on the other hand can verify and improve the accuracy of the experimental results. A positive control in an ELISA test can be considered the standard product. Negative control is a blank matrix or a matrix solution with known low concentration that has homology and homogeneity with the object to be picked up, which is generally difficult to obtain, and can be set as a negative control if available. The routine is to set a blank control, that is, a sample diluent.
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Q7:Why should a product with two item numbers of a target test different sample types?
Different sample types of matrix are different, in order to reduce the influence of the matrix itself and improve the accuracy of routine sample measurement, usually set different systems of ELISA kits, and display with different product numbers. In addition, the concentration of some indicators varies greatly between different samples, so different products are used to meet customers' needs for ease of operation.
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Q8:How can I tell if my sample needs to be diluted or processed?
The detection range of the kit is not the same as the concentration range of the substance to be measured in the sample. It is recommended to estimate the concentration of the substance to be measured in the sample through relevant literature before the experiment and determine the actual concentration of the sample through pre-experiment. If it is a routine sample type, you can consult ELISA technical support to obtain the recommended dilution of the sample and schedule pre-laboratory testing. If the concentration of the substance to be measured in the sample is too high or too low, dilute or concentrate the sample appropriately. If the concentration of analyte in the sample is much lower than the minimum detection line of the kit, it is recommended to choose a kit with higher sensitivity for detection.
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Q9:Is it necessary to set up multiple Wells for ELISA?
In order to ensure the accuracy of the experimental results, we suggest that both standards and samples should be tested in duplicate. Of course, ELISA experiments do not necessarily have to be repeated /3 repeated Wells, customers can set the repeat or not according to their own needs.
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Q10:How to perform ultrasonic processing?
Using ultrasonic cell crusher, ultrasonic treatment of the suspension to lyse the cells (reference: Soniprep150 ultrasonic generator, ultrasonic treatment of 30s at an amplitude of 14μm, cell fragmentation; Or use an ultrasonic crusher, 200W, 2s/ times, gap 3s, total time 3-5min or 400 amp, 5s/ times, gap 10s, repeated 3-5 times), and then centrifuge at 2 to 8℃ at 1500×g for 10min, remove the cell debris, collect the supernatant. Attention should be paid to temperature during ultrasound.